In the present work, simple, sensitive, specific, and validated colorimetric method has been developed for the quantitative estimation of Voriconazole in bulk and pharmaceutical dosage form. Voriconazole in presence of acidic medium reacts with excess amount of Chloramine-T and unreacted Chloramine-T reacts with crystal violet to produce blue colour. The λmax was found to be 580 nm for assay. The linearity was found in concentration range of 5-30 µg/ml. The correlation coefficient was found 0.9992. The regression equation was found as Y= 0.020 X + 0.00. The method was validated for linearity, accuracy, precision and ruggedness. The LOD and LOQ for estimation of voriconazole were found as 0.02250 & 0.06819 respectively. Recovery of Voriconazole was found to be 98.73 %. Proposed method was successfully applied for the quantitative estimation of voriconazole in marketed formulations.
Loading....